Gene Synthesis /
Molecular Biology

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GENE SYNTHESIS
Standard Genes
Express Genes
Complex Genes
Combinatorial Libraries
Gene Synthesis Project
Gene Fragments = GeneStrands
Gene Strands
Express GeneStrands
MOLECULAR BIOLOGY SERVICES
Plasmid Preparation
Site Directed Mutagenesis
DNA Cloning
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Corona Control Plasmids
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PUBLICATIONS

Selection of publications by our customers who are using our gene synthesis & molecular biology

Gene synthesis: Di Nitto C, Neri D, Weiss T, Weller M, De Luca R. Design and Characterization of Novel Antibody-Cytokine Fusion Proteins Based on Interleukin-21. Antibodies. 2022

GeneStrands: Forrest, D., Warman, E.A., Erkelens, A.M. et al. Xenogeneic silencing strategies in bacteria are dictated by RNA polymerase promiscuity. Nat Commun

Gene synthesis: Liu et al. Bivalent single domain antibody constructs for effective neutralization of Venezuelan equine encephalitis. Scientific reports

Gene synthesis: Reimer et al. Engineering the amoeba Dictyostelium discoideum for biosynthesis of a cannabinoid precursor and other polyketides. Nature Biotechnology

 

SARS-CoV-2 research: Signorini et al. Longitudinal, virological, and serological assessment of hospitalized COVID-19 patients

SARS-CoV-2 research: Monteiro et al. Recovery of SARS-CoV-2 from large volumes of raw wastewater is enhanced with the inuvai R180 system. Journal of Environmental Management

SARS-CoV-2 research: Herrlein et al. Comparative Investigation of Methods for Analysis of SARS-CoV-2-Spike-Specific Antisera. Viruses

SARS-CoV-2 research: Anna et. al., High seroprevalence but short‐lived immune response to SARS‐CoV‐2 infection in Paris

SARS-CoV-2 research: Rajasekharan et. al., Inhibitors of Protein Glycosylation Are Active against the Coronavirus Severe Acute Respiratory Syndrome Coronavirus SARS-CoV-2

SARS-CoV-2 research: Bechtold et. al., Rapid Identification of SARS-CoV-2 Variants of Concern Using a Portable peakPCR Platform

 

Gene synthesis: Marusic, et al., The expression in plants of an engineered VP2 protein of Infectious Bursal Disease Virus induces formation of structurally heterogeneous particles that protect from a very virulent viral strain

Gene synthesis: Shams, et al., Evaluation of the Binding Kinetics of RHEB with mTORC1 by In-cell and In vitro Assays

Gene synthesis: Unno, et al., Heterologous expression of cryptic biosynthetic gene cluster from Streptomyces prunicolor yields novel bicyclic peptide prunipeptin

Gene synthesis: Nakazato, et al., Targeted base editing in the plastid genome of Arabidopsis thaliana

Gene synthesis: Marine, et al., Towards unravelling the Rosette agent enigma: Spread and emergence of the co-invasive host-pathogen complex, Pseudorasbora parva-Sphaerothecum destruens

Gene synthesis: Johari, et al., Design of synthetic promoters for controlled expression of therapeutic genes in retinal pigment epithelial cells

Gene synthesis: Wang, et al., A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections

Gene synthesis: Wong T.S., Tee K.L. (2020) Gene Cloning. In: A Practical Guide to Protein Engineering. Learning Materials in Biosciences. Springer, Cham

SARS-CoV-2 research: Ruiz-Arrondo., et al., Detection of SARS‐CoV‐2 in pets living with COVID‐19 owners diagnosed during the COVID‐19 lockdown in Spain: A case of an asymptomatic cat with SARS‐CoV‐2 in Europe

Gene synthesis: Ferrari et al., The allergen Mus m 1.0102: Cysteine residues and molecular allergology, Molecular Immunology

Gene synthesis: Fernandes et al., The dimerisable Cre recombinase allows conditional genome editing in the mosquito stages of Plasmodium berghei

Gene synthesis: Abu-Halima M., et al., Differential expression of miR-23a/b-3p and its target genes in male patients with subfertility

Gene synthesis: Johari Y. B., et al., CHO genome mining for synthetic promotor design, 

Gene synthesis: Sota M., et al., Efficient transposition of Tn4556 by alterations in inverted repeats using a delivery vector carrying a counter-selectable marker for Streptomyces

Gene synthesis: Jimmy S., et al., Discovery of small alarmone synthetases and their inhibitors as toxin-antitoxin loci

Gene synthesis: Liu J., et al., Polychromatic solar energy conversion in pigment-protein chimeras that unite the two kingdoms of (bacterio)chlorophyll-based photosynthesis

Gene synthesis: Tebo AG., et al., A plit fluorescent reporter with rapid and reversible complementation

Gene synthesis: Jaubert, C. et al., RNA synthesis is modulated by G-quadruplex formation in Hepatitis C virus negative RNA strand

Gene synthesis: Fusco, F. A. et al., Biochemical charaterization of a thermostable endomannanase / endoglucanase from Dictyoglomus turgidum

Gene synthesis: Bernardini A. et al., The phosphorylatable Ser320 of NF-YA is involved in DNA binding of the NF-Y trimer

Gene synthesis: Amiri, M. M. et al., Hersintuzumab: A novel humanized anti-HER2 monoclonal antibody induces potent tumor growth inhibition

Gene synthesis: Fathi, S. et al., Design and construction of a new recombinant fusion protein (2b2t+EPC1) and its assessment for serodiagnosis of cystic echinococcosis

Gene synthesis: Brenner, N. et al., Validation of Multiplex Serology detecting human herpesviruses 1-5

Gene synthesis: Nolvak, H. et al., Reduction of antibiotic resistome and integron-integrase genes in laboratory scale photobioreactors treating municipal wastewater

Gene synthesis: Chow J., et al., The Thaumarchaeon N. gargensis carries functional bioABD genes and has a promiscuous E. coli deltabioH-complementing esterase EstN1

Gene synthesis: Le, T, M. et al., Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain.

Gene synthesis: Mekonnen S. et al., O-5S quantitative real-time PCR: a new diagnostic tool for laboratory confirmation of human onchocerciasis

Gene synthesis: Wingen, M. et al., Novel Thermostable Flavin‐binding Fluorescent Proteins from Thermophilic Organisms

Gene synthesis: Nolvak H., et al., Inorganic and organic fertilizers impact the abundance and proportion of antibiotic resistance and integron-integrase genes in agricultural grassland soil

Gene synthesis: Whitten MM., et al., Symbiont-mediated RNA interference in insects

Gene synthesis: Ohno M., et al., Acidic mammalian chitinase is a proteases-resistant glycosidase in mouse digestive system

Gene synthesis: Hoshiba T., Decellularized matrices as in vitro models of extracellular matrix in tumor tissues at different malignant levels: Mechanism of 5-fluorouracil resistance in colorectal tumor cells

Gene synthesis: Turner K.B., et al., Next-Generation Sequencing of a Single Domain Antibody Repertoire Reveals Quality of Phage Display Selected Candidates

Gene synthesis: Sudo Y., et al., Functional and Photochemical Characterization of a Light‐Driven Proton Pump from the Gammaproteobacterium Pantoea vagans

Gene synthesis: Scorsone E., et al., Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction

Gene synthesis: Brieke, C. et al., Rapid access to glycopeptide antibiotic precursor peptides coupled with cytochrome P450-mediated catalysis: towards a biomimetic synthesis of glycopeptide antibiotics. Organic & biomolecular chemistry

Gene synthesis: Takeishi, R. et al., Epimerization at C-3′′ in Butirosin Biosynthesis by an NAD+-Dependent Dehydrogenase BtrE and an NADPH-Dependent Reductase BtrF. ChemBioChem

Gene synthesis: Buettner, K. et al., Protein surface charge of trypsinogen changes its activation pattern. BMC biotechnology 14(1)

Gene synthesis: Chiwata, R. et al., None of the Rotor Residues of F 1-ATPase Are Essential for Torque Generation. Biophysical journal 106(10)

Gene synthesis: Holmberg, M. et al., A versatile bacterial expression vector designed for single-step cloning of multiple DNA fragments using homologous recombination. Protein expression and purification 98

Gene synthesis: Dietz O. et al., Characterization of the small exported Plasmodium falciparum membrane protein SEMP1. PLOS ONE 9(7): e103272

Gene synthesis: Canali E. et al., A high-performance thioredoxin-based scaffold for peptide immunogen construction: proof-of-concept testing with a human papillomavirus epitope. Nature Scientific Reports 4(4729)

Gene synthesis: Mueller W. et al., Hierarchical organization of multi-site phosphorylation at the CXCR4 C Terminus, PLOS ONE 8(5): e64975

Gene synthesis:
 Van Noort, V., Bradatsch, B., Arumugam, M., Amlacher, S et al.,  Consistent mutational paths predict eukaryotic thermostability. BMC Evolutionary Biology, 13(1), 7

Gene synthesis: Carciofi M. et al. Concerted suppression of all starch branching enzyme genes in barely produces amylose-only starch granules. BMC Plant Biology 2012, 12:223

Gene synthesis: Lejonklou M. et al. Accelerated Proliferation and Differential Global Gene Expression in Pancreatic Islets of Five-Week-Old Heterozygous Men1 Mice: Men1 Is a Haploinsufficient Suppressor. Endocrinology April 2012 (Genes purchase/synthesis: kinase complex-associated protein (Ikbkap)

Cloning: Ullrich T. et al. Competitive Reporter Monitored Amplification (CMA) – Quantification of Molecular Targets by Real Time Monitoring of Competitive Reporter Hybridization. PLoS ONE 7(4): e35438. (IPC gene for HIV- 1 and HIV- 2 respectively cloned into the pCR 2.1 TOPO® vector)

Gene synthesis: Ritari J. et al. Detection of Human Papillomaviruses by Polymerase Chain Reaction and Ligation Reaction on Universal Microarray. PLoS ONE 7(3): e34211. (HPV sequences were synthesised and cloned into plasmid vector)

Site-directed mutatgenesis: Esposito F. et al. Down-Regulation of the miR-25 and miR-30d Contributes to the Development of Anaplastic Thyroid Carcinoma Targeting the Polycomb Protein EZH2. Journal of Clincial Endocrinology & Metabolism May 1, 2012 vol. 97 no. 5 E710-E718

Gene synthesis: Weber E. et al. Assembly of Designer TAL Effectors by Golden Gate Cloning. PLoS ONE 6(5): e19722

Gene synthesis: Gays F. et al. Comprehensive Analysis of Transcript Start Sites in Ly49 Genes Reveals an Unexpected Relationship with Gene Function and a Lack Of Upstream Promoters. PLoS ONE 6(3): e18475.

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